Description
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with TNF-alpha. Standards and samples are pipetted into the wells with a biotin-conjugated TNF-alpha and avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNF-alpha antibody bound in the initial step. The color development is stopped and the intensity of the color is measured